Anti-AFP (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P02771
Gene Id	174
Gene Symbols	AFP
Protein Name / Synonyms	AFP (Center)
Clonality	Polyclonal
Preparation	This AFP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 amino acids from the Central region of human AFP.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Specificity

Other Names

AFP; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin

NCBI Accession #

NP_001125.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

68678

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50

	Western blot analysis of AFP (arrow) using rabbit polyclonal AFP Antibody (Center) (Cat# 102-12422). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AFP gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with AFP antibody (Center) (Cat# 102-12422), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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