Synonyms
D-AKAP2 antibody, MGC9414 antibody, PRKA10 antibody, AKAP10 antibody, dual specificity A kinase-anchoring protein 2 antibody, D-AKAP-2 antibody, a kinase anchor protein 10, mitochondrial antibody, A-kinase anchor protein 10, mitochondrial antibody, dual-specificity A-kinase anchoring protein 2 antibody, protein kinase A anchoring protein 10 antibody, protein kinase A-anchoring protein 10 antibody, AKAP-10 antibody, mitochondrial A kinase PPKA anchor protein 10 antibody, A kinase (PRKA) anchor protein 10 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
74 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 600 and 662 of AKAP10 (Uniprot ID# O43572)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections)
Application Notes
Suggested starting dilutions are as follows: IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using AKAP10(107-10074) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded T(gastric CA), using AKAP10(107-10074) antibody at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded OVCAR3, using AKAP10(107-10074) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.