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Anti-AKAP12 (C-term) Antibody
Catalog #: 107-10615
AKAP12 antibody [C3], C-Terminus, 0.73 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 9590 |
| Protein Name / Synonyms | AKAP12, C-term |
| Concentration (lot specific) | 0.73 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
AKAP250 antibody, DKFZp686M0430 antibody, DKFZp686O0331 antibody, FLJ20945 antibody, FLJ97621 antibody, AKAP12 antibody, Src-Suppressed C Kinase Substrate antibody, "A-kinase anchor protein, 250kDa antibody", myasthenia gravis autoantigen gravin antibody, A-kinase anchor protein 12 antibody, kinase scaffold protein gravin antibody, AKAP 250 antibody, A kinase (PRKA) anchor protein 12 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
191 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1719 and 1782 of AKAP12 (Uniprot ID# Q02952)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AKAP12(107-10615) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded serous OVCA, using AKAP12(107-10615) antibody at 1:100 dilution.
Sample (30µg whole cell lysate)
A:HeLa S3
5% SDS PAGE
107-10615 diluted at 1:1000
A:HeLa S3
5% SDS PAGE
107-10615 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.