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Anti-AKR1B1 Antibody
Catalog #: 144-60529
AKR1B1 Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P15121 |
| Gene Id | 231 |
| Gene Symbols | AKR1B1 |
| Protein Name / Synonyms | AKR1B1, ADR, ALDR1, ALR2, AR, aldose reductase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
35kDa
Observed MW
36.5kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human AKR1B1 (NP_001619.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using AKR1B1 antibody (144-60529) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat lung using AKR1B1 Antibody (144-60529) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using AKR1B1 Antibody (144-60529) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.