Anti-AKR1C1 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	1645
Protein Name / Synonyms	AKR1C1
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunohistochemistry, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

2-ALPHA-HSD antibody, 20-ALPHA-HSD antibody, C9 antibody, DD1 antibody, DDH antibody, DDH1 antibody, H-37 antibody, HAKRC antibody, MBAB antibody, MGC8954 antibody, AKR1C1 antibody, 20-alpha-hydroxysteroid dehydrogenase antibody, dihydrodiol dehydrogenase 1/2 antibody, trans-1,2-dihydrobenzene-1,2-diol dehydrogenase antibody, high-affinity hepatic bile acid-binding protein antibody, dihydrodiol dehydrogenase isoform DD1 antibody, indanol dehydrogenase antibody, aldo-keto reductase family 1 member C1 antibody, aldo-keto reductase C antibody, type II 3-alpha-hydroxysteroid dehydrogenase antibody, chlordecone reductase homolog HAKRC antibody, hepatic dihydrodiol dehydrogenase antibody, 20 alpha-hydroxysteroid dehydrogenase antibody, dihydrodiol dehydrogenase 1 antibody, aldo-keto reductase family 1, member C1 (dihydrodiol dehydrogenase 1, 20-alpha (3-alpha)-hydroxysteroid dehydrogenase) antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

37 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 22 and 248 of AKR1C1 (Uniprot ID# Q04828)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunohistochemistry,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed A431, using AKR1C1(107-10539) antibody at 1:200 dilution.

AKR1C1 antibody detects AKR1C1 protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
10 % SDS-PAGE
AKR1C1 antibody (107-10539) dilution: 1:1000

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft , using AKR1C1(107-10539) antibody at 1:500 dilution.

Sample(30 ug whole cell lysate)
A:Raji
10% SDS PAGE
107-10539 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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