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Anti-AKT1 Antibody
Catalog #: 144-61491
Phospho-AKT1-S473 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P31749 |
| Gene Id | 207 |
| Gene Symbols | AKT1 |
| Protein Name / Synonyms | AKT1, AKT, CWS6, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA, AKT serine/threonine kinase 1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IP |
| Research Area | Angiogenesis, Apoptosis, Autophagy, Akt Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene.
Dilutions
WB 1:500 - 1:2000
IP 1:50 - 1:100
IP 1:50 - 1:100
Format
Calculated MW
48kDa/55kDa
Observed MW
60kDa
Immunogen
A synthetic phosphorylated peptide around S473 of human AKT1 (NP_005154.2).
Modification
Phospho S473
Storage
-20°C
Images
Western blot analysis of extracts of Jurkat cells,using Phospho-AKT1-S473 antibody (144-61491) at 1:2000 dilution. Jurkat cells were treated by Calyculin A (100nM) for 30 minutes.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug Phospho-AKT1-S473 pAb (144-61491). Western blot was performed from the immunoprecipitate using Phospho-AKT1-S473 pAb (144-61491) at a dilition of 1:1000. Jurkat cells were treated by Calyculin A (100 nM) at 37? for 30 minutes.
Expiration:
12 months from the date of shipment when stored properly.