Rabbit Anti-Human AKT1 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P31749 |
| Gene Id | 207 |
| Gene Symbols | AKT1 |
| Protein Name / Synonyms | AKT1 (C-term) |
| Clonality | Polyclonal |
| Preparation | This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-468 amino acids from the C-terminal region of human AKT1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, FC |
| Research Area | Angiogenesis, Apoptosis, Autophagy, Akt Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.
Specificity
Predicted Reactivity
Mouse, Rat
Other Names
AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; Protein kinase B alpha; Proto-oncogene c-Akt; RAC-PK-alpha
NCBI Accession #
NP_001014431.1;NP_001014432.1;NP_005154.2
Other Accession #
P47196; P31750
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
55686
Recommended Dilutions
WB: 1:1000; FC: 1:10-50
 | Western blot analysis of hAKT1-D453 (Cat# 102-17081) in Y79 cell line lysates (35ug/lane).AKT1 (arrow) was detected using the purified Pab. |
 | Western blot analysis of AKT1 (arrow) using rabbit polyclonal hAKT1-D453 (Cat# 102-17081). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT1 gene (Lane 2) (Origene Technologies). |
 | AKT1 Antibody (C-term) (Cat# 102-17081) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the AKT1 antibody detected the AKT1 protein (arrow). |
 | AKT1 Antibody (C-term) (Cat# 102-17081) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.