Anti-AKT1 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P31749
Gene Id	207
Gene Symbols	AKT1
Protein Name / Synonyms	AKT1 (N-term)
Clonality	Polyclonal
Preparation	This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-76 amino acids from the N-terminal region of human AKT1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IF, FC
Research Area	Angiogenesis, Apoptosis, Autophagy, Akt Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

AKT1; PKB; RAC; RAC-alpha serine/threonine-protein kinase; Protein kinase B; Protein kinase B alpha; Proto-oncogene c-Akt; RAC-PK-alpha

NCBI Accession #

NP_001014431.1;NP_001014432.1;NP_005154.2

Other Accession #

P47196; P31750; Q01314

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

55686

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of AKT1 Antibody (N-term) (Cat# 102-17146) in MCF-7 cell line lysates (35ug/lane). AKT1 (arrow) was detected using the purified Pab.
	Confocal immunofluorescent analysis of AKT1 Antibody (N-term)(Cat# 102-17146) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	AKT1 Antibody (N-term) (Cat# 102-17146) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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