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Anti-AKT1S1 Antibody
Catalog #: 144-61523
Phospho-AKT1S1-T246 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q96B36 |
| Gene Id | 84335 |
| Gene Symbols | AKT1S1 |
| Protein Name / Synonyms | AKT1S1, Lobe, PRAS40, AKT1 substrate 1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
13kDa/27kDa/29kDa
Observed MW
45kDa
Immunogen
A synthetic phosphorylated peptide around T246 of human AKT1S1 (NP_001092102.1).
Modification
Phospho T246
Storage
-20°C
Images
Western blot analysis of extracts of HeLa cells, using Phospho-AKT1S1-T246 antibody (144-61523) at 1:2000 dilution. HeLa cells were treated by Insulin (0.01U/ml) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.