Anti-AKT2 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P31751
Gene Id	208
Gene Symbols	AKT2
Protein Name / Synonyms	AKT2 (C-term)
Clonality	Polyclonal
Preparation	This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 416-444 amino acids from the C-terminal region of human AKT2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cancer, Akt Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

AKT2 is a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. AKT2 has been shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.

Specificity

Predicted Reactivity

Mouse, Rat

Other Names

AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; RAC-PK-beta

NCBI Accession #

NP_001617.1

Other Accession #

P47197; Q60823

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

55769

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Fluorescent confocal image of C2C12 cell stained with AKT2 Antibody (C-term)(Cat# 102-17083).C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AKT2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).AKT2 immunoreactivity is localized to Cytoplasm significantly.
	Western blot analysis of AKT2 (arrow) using AKT2 Antibody (C-term) (Cat# 102-17083). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AKT2 gene (Lane 2) (Origene Technologies).
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AKT2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	AKT2 Antibody (C-term) (Cat# 102-17083) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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