Anti-AKT2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P31751
Gene Id	208
Gene Symbols	AKT2
Protein Name / Synonyms	AKT2 (N-term)
Clonality	Polyclonal
Preparation	This AKT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the N-terminal region of human AKT2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cancer, Akt Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

AKT2 is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. Furthermore, AKT2 was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression of AKT2 contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. AKT2 is a general protein kinase capable of phophorylating several known proteins.

Specificity

Other Names

AKT2; RAC-beta serine/threonine-protein kinase; Protein kinase Akt-2; Protein kinase B beta; RAC-PK-beta

NCBI Accession #

NP_001617.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

55769

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of anti-AKT2 Antibody (N-term) Pab (Cat# 102-17082) in mouse liver tissue lysates (35ug/lane). AKT2(arrow) was detected using the purified Pab.
	Western blot analysis of anti-AKT2 Antibody (N-term) Pab (Cat# 102-17082) in 293 cell line lysates transiently transfected with the AKT2 gene (2ug/lane). AKT2(arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human colon carcinoma reacted with AKT2 Antibody (N-term)(Cat# 102-17082), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of AKT2 Antibody (N-term)(Cat# 102-17082) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
	AKT2 Antibody (N-term) (Cat# 102-17082) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.