Anti-ALDH1A1 Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Accession Number	P00352
Gene Id	216
Gene Symbols	ALDH1A1
Protein Name / Synonyms	ALDH1A1
Clonality	Monoclonal
Preparation	This ALDH1A1 monoclonal antibody is generated from mouse immunized with ALDH1A1 recombinant protein.
Concentration (lot specific)	0.320 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ALDH1A1 encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis.

Specificity

Other Names

ALDH1A1; ALDC; ALDH1; PUMB1; Retinal dehydrogenase 1; ALDH-E1; ALHDII; Aldehyde dehydrogenase family 1 member A1; Aldehyde dehydrogenase, cytosolic

NCBI Accession #

NP_000680.2

Other Accession #

NP_000680.2

Format

Type

Purified Mouse Monoclonal Antibody (Mab)

Calculated Molecular Weight (Da)

54862

Recommended Dilutions

IF: 1:10-50; WB: 1:500-1000; IHC: 1:50-100

	Fluorescent confocal image of HepG2 cells stained with ALDH1A1 antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-10069 ALDH1A1 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). ALDH1A1 immunoreactivity is localized to the cytoplasm of HepG2 cells.
	ALDH1A1 Monoclonal Antibody(Cat# 102-10069) western blot analysis in NCI-H460 cell line lysates (15ug/lane).This demonstrates the ALDH1A1 antibody detected the ALDH1A1 protein (arrow).
	ALDH1A1 Monoclonal Antibody (Cat# 102-10069) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ALDH1A1 Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of ALDH1A1 Antibody (Cat# 102-10069) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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