Anti-ALG2 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9H553
Gene Id	85365
Gene Symbols	ALG2
Protein Name / Synonyms	ALG2, CDG1I, CDGIi, CMS14, CMSTA3, NET38, hALPG2, alpha-1,3/1,6-mannosyltransferase ALG2
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC
Research Area	Autoimmune Disease
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Alternative splicing results in multiple transcript variants.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW

37kDa/47kDa

Observed MW

47kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 197-416 of human ALG2 (NP_149078.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using ALG2 antibody (144-07843) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - ALG2 antibody (144-07843)

Immunohistochemistry of paraffin-embedded human liver cancer using ALG2 Antibody (144-07843) at dilution of 1:100 (40x lens).
Immunohistochemistry - ALG2 Antibody (144-07843)

Expiration:

12 months from the date of shipment when stored properly.

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