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Anti-Alkaline phosphatase (ALPP) Antibody

Catalog #: 130-10128

Rabbit Anti-Alkaline phosphatase( ALPP) Antibody

Product Description

Specifications

Size20 µg, 100 µg, 200 µg, 500 µg
Estimated Lead Time1-2 business days
SpeciesHuman
Accession Number
AAH09647
ClonalityPolyclonal
Immunogen
The imunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography.
Recommended ApplicationsELISA (recommended work dilution= 1: 128, 000) , Western Blotting (recommended work dilution= 1: 1, 000-5, 000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein ALPP. The membrane blot was probed with antiALPP primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein ALPP from E.coli cell (57kDa).
Antigen Catalog Number
230-00257
Reconstitution
Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Shipping TypeAmbient temperature
Storage-20°C

Description

Description

Alkaline Phosphatase(ALPP), placental type is a membrane bound glycosylated dimeric enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. It is a tumor marker, especially in seminoma and ovarian cancer.

Typical Data

Cell Lysate: SAOS2 0.2 mg/ml | Secondary Ab: Rabbit | Exposure: 30 sec | Expected MW (kDa): 55

Cell Lysate: Hela 0.2 mg/ml | Secondary Ab: Rabbit | Exposure: 30 sec | Expected MW (kDa): 55

Cell Lysate: HEPG2 0.2 mg/ml | Secondary Ab: Rabbit | Exposure: 30 sec | Expected MW (kDa): 55

Cell Lysate: 143B 0.2 mg/ml | Secondary Ab: Rabbit | Exposure: 30 sec | Expected MW (kDa): 55

Specificity

This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat ALPP has not been tested yet.

Storage/Stability

Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.

Expiration

12 months from the date of shipment when stored properly.

References

  • Dimitra Georgiadou, et al. PLAP efficiently generates mature antigenic peptides in vitro but in patterns distinct from ERAP1. J Immunol. 2010 August 1; 185(3): 1584–1592.
  • Lindsay A. Farrer, et al. Waardenberg syndrome (WS) type I is caused by defects at multiple loci, one of which is near ALPP on chromosome 2: First report of the WS consortium. Am J Hum Genet. 1992 May; 50(5): 902–913.