Rabbit anti-Alkaline phosphatase (ALPP) Biotinylated Antibody, 50 ug
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Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | AAH09647 |
| Clonality | Polyclonal |
| Immunogen | The imunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 128, 000) , Western Blotting (recommended work dilution= 1: 1, 000-5, 000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein ALPP. The membrane blot was probed with antiALPP primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein ALPP from E.coli cell (57kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Alkaline Phosphatase(ALPP), placental type is a membrane bound glycosylated dimeric enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. It is a tumor marker, especially in seminoma and ovarian cancer.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat ALPP has not been tested yet.
Related Products
Native Human Alkaline Phosphatase, Placental (MD-14-0001P) Mouse Anti-Human Placental Alkaline Phosphatase (MD-15-0115)
References
- Dimitra Georgiadou, et al. PLAP efficiently generates mature antigenic peptides in vitro but in patterns distinct from ERAP1. J Immunol. 2010 August 1; 185(3): 15841592.
- Lindsay A. Farrer, et al. Waardenberg syndrome (WS) type I is caused by defects at multiple loci, one of which is near ALPP on chromosome 2: First report of the WS consortium. Am J Hum Genet. 1992 May; 50(5): 902913.
Expiration:
12 months from the date of shipment when stored properly.
