Rabbit Anti-Human ALOX12 (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P18054 |
| Gene Id | 239 |
| Gene Symbols | ALOX12 |
| Protein Name / Synonyms | ALOX12 (C-term) |
| Clonality | Polyclonal |
| Preparation | This ALOX12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 618-650 amino acids from the C-terminal region of human ALOX12. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF, FC |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Oxygenase and 14,15-leukotriene A4 synthase activity.
Specificity
Other Names
ALOX12; LOG12; Arachidonate 12-lipoxygenase, 12S-type; Platelet-type lipoxygenase 12
NCBI Accession #
NP_000688.2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
75694
Recommended Dilutions
IF: 1:100; WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Immunofluorescent analysis of A549 cells, using ALOX12 Antibody (C-term) (Cat# 102-18063). 102-18063 was diluted at 1?100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor® 554 (red) conjugated Phalloidin (red). |
 | Western blot analysis of ALOX12 Antibody (C-term) (Cat# 102-18063) in K562 cell line lysates (35ug/lane). ALOX12 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human skin tissue reacted with ALOX12 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | ALOX12 Antibody (C-term) (Cat# 102-18063) flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.