alpha 1a Adrenergic Receptor antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 148 |
| Protein Name / Synonyms | alpha 1a Adrenergic Receptor |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ADRA1C antibody, ADRA1L1 antibody, ALPHA1AAR antibody, ADRA1A antibody, "adrenergic, alpha-1A-, receptor variant 3 antibody", G protein coupled receptor antibody, "adrenergic, alpha-1A-, receptor variant 8 antibody", alpha-1A adrenoreceptor antibody, alpha-1C adrenergic receptor antibody, "adrenergic, alpha-1A-, receptor variant 5 antibody", alpha-1A adrenoceptor antibody, "adrenergic, alpha-1A-, receptor variant 1 antibody", alpha-1A adrenergic receptor antibody, "adrenergic, alpha-1A-, receptor antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
1XPBS, 1%BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
51 kDa
Immunogen
Synthetic peptide contain a sequence corresponding to a region within amino acids 206 and 260 (P35348) of alpha 1a Adrenergic Receptor
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed Hep3B, using alpha 1a Adrenergic Receptor(107-10189) antibody at 1:500 dilution.
Immunohistochemical analysis of paraffin-embedded Heart, using alpha 1a Adrenergic Receptor(107-10189) antibody(10 µg/ml).
alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein by western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
alpha 1a Adrenergic Receptor antibody (107-10189) dilution: 1:1000
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
C. 30 µg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
alpha 1a Adrenergic Receptor antibody (107-10189) dilution: 1:1000
alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
alpha 1a Adrenergic Receptor antibody (107-10189) dilution: 1:500.
Sample: Paraffin-embedded mouse prostate.
alpha 1a Adrenergic Receptor antibody (107-10189) dilution: 1:500.
alpha 1a Adrenergic Receptor antibody detects alpha 1a Adrenergic Receptor protein at cytosol on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
alpha 1a Adrenergic Receptor antibody (107-10189) dilution: 1:500.
Sample: Paraffin-embedded rat hind brain.
alpha 1a Adrenergic Receptor antibody (107-10189) dilution: 1:500.
Sample(30 µg of whole cell lysate)
A:Raji
10% SDS PAGE
107-10189 diluted at 1:1000
A:Raji
10% SDS PAGE
107-10189 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.