Synonyms
A2AP antibody, AAP antibody, ALPHA-2-PI antibody, API antibody, PLI antibody, SERPINF2 antibody, serpin F2 antibody, alpha-2-plasmin inhibitor antibody, alpha-2-AP antibody, alpha-2-antiplasmin antibody, "serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2 antibody", "serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 2 antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
55 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 188 and 453 of alpha 2 Antiplasmin (Uniprot ID# P08697)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed A431, using SERPINF2(107-10895) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using alpha 2 Antiplasmin(107-10895) antibody at 1:500 dilution.

Sample (50 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
107-10895 diluted at 1:1000

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
107-10895 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.