alpha Actin (cardiac muscle) antibody, 0.7 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 70 |
| Protein Name / Synonyms | alpha Actin (cardiac muscle) |
| Concentration (lot specific) | 0.7 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Cardiovascular Disease, Cytoskeletal Reorganization |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ACTC antibody, ASD5 antibody, CMD1R antibody, CMH11 antibody, LVNC4 antibody, ACTC1 antibody, "actin, alpha cardiac muscle 1 antibody", alpha-cardiac actin antibody, "actin, alpha, cardiac muscle 1 antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
42 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 99 and 354 (P68032) of alpha Actin (cardiac muscle)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Gastric CA, using alpha Actin (cardiac muscle)(107-10297) antibody at 1:100 dilution.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
107-10297 diluted at 1:1000
A: NIH-3T3
10% SDS PAGE
107-10297 diluted at 1:1000
alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
alpha Actin (cardiac muscle) antibody (107-10297) dilution: 1:10000
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
10 % SDS-PAGE
alpha Actin (cardiac muscle) antibody (107-10297) dilution: 1:10000
alpha Actin (cardiac muscle) antibody detects alpha Actin (cardiac muscle) protein at cytoskeleton by immunofluorescent analysis.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: alpha Actin (cardiac muscle) protein stained by alpha Actin (cardiac muscle) antibody (107-10297) diluted at 1:200.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in 0.5% Triton X-100 for 1 min, then ice-cold methanol for 5 min.
Green: alpha Actin (cardiac muscle) protein stained by alpha Actin (cardiac muscle) antibody (107-10297) diluted at 1:200.
Blue: Hoechst 33342 staining.
Sample(30 µg of whole cell lysate)
A:HeLa S3
B:MOLT4
12% SDS PAGE
107-10297 diluted at 1:500
A:HeLa S3
B:MOLT4
12% SDS PAGE
107-10297 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.