Synonyms
ACTA antibody, ASMA antibody, CFTD antibody, CFTD1 antibody, CFTDM antibody, MPFD antibody, NEM1 antibody, NEM2 antibody, NEM3 antibody, ACTA1 antibody, alpha-actin-1 antibody, alpha skeletal muscle actin antibody, "actin, alpha skeletal muscle antibody", "actin, alpha 1, skeletal muscle antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
42 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 377 of alpha Actin (skeletal muscle)
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Skeletal Muscle , using alpha Actin (skeletal muscle)(107-10257) antibody(10 µg/ml).

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
D: C2C12
10% SDS PAGE
107-10257 diluted at 1:5000

Sample(30 µg of whole cell lysate)
A:Raji
10% SDS PAGE
107-10257 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.