Anti-alpha Tubulin 1A Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat, Drosophila
Host Species	Rabbit
Gene Id	7846
Protein Name / Synonyms	alpha Tubulin 1A
Concentration (lot specific)	0.91 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Cytoskeletal Reorganization
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

B-ALPHA-1 antibody, FLJ25113 antibody, LIS3 antibody, TUBA3 antibody, TUBA1A antibody, tubulin B-alpha-1 antibody, hum-a-tub1 antibody, "tubulin, alpha, brain-specific antibody", hum-a-tub2 antibody, tubulin alpha-1A chain antibody, "tubulin, alpha 3 antibody", alpha-tubulin 3 antibody, tubulin alpha-3 chain antibody, "tubulin, alpha 1a antibody"

Specificity

Species Reactivity

Human, Rat, Mouse, Drosophila

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

50 kDa

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 37 and 276 of alpha Tubulin 1A

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:20000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using alpha Tubulin 1A(107-10871) antibody at 1:500 dilution.

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 30 µg drosophila lysate/extract
10 % SDS-PAGE
alpha Tubulin 1A antibody (107-10871) dilution: 1:1000

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 30 µg NIH-3T3 whole cell extract
B. 30 µg mouse brain extract
10 % SDS-PAGE
alpha Tubulin 1A antibody (107-10871) dilution: 1:10000

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 30 µg Jurkat whole cell extract
B. 30 µg Raji whole cell extract
C. 30 µg 293T whole cell extract
D. 30 µg A431 whole cell extract
E. 30 µg HeLa whole cell extract
F. 30 µg HepG2 whole cell extract
G. 30 µg H1299 whole cell extract
H. 30 µg HCT116 whole cell extract
I. 30 µg MCF-7 whole cell extract
J. 30 µg NT2D1 whole cell extract
K. 30 µg PC-3 whole cell extract
L. 30 µg U87-MG whole cell extract
10 % SDS-PAGE
alpha Tubulin 1A antibody (107-10871) dilution: 1:10000

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein by western blot analysis.
A. 30 µg rat brain extract
10 % SDS-PAGE
alpha Tubulin 1A antibody (107-10871) dilution: 1:10000

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
10% SDS PAGE
107-10871 diluted at 1:10000

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Tubulin alpha 1A(107-10871) antibody at 1:200 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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