Anti-alpha Tubulin 1A Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	7846
Protein Name / Synonyms	alpha Tubulin 1A
Concentration (lot specific)	0.24 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Cytoskeletal Reorganization
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

B-ALPHA-1 antibody, FLJ25113 antibody, LIS3 antibody, TUBA3 antibody, TUBA1A antibody, tubulin B-alpha-1 antibody, hum-a-tub1 antibody, "tubulin, alpha, brain-specific antibody", hum-a-tub2 antibody, tubulin alpha-1A chain antibody, "tubulin, alpha 3 antibody", alpha-tubulin 3 antibody, tubulin alpha-3 chain antibody, "tubulin, alpha 1a antibody"

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

50 kDa

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 227 and 440 (Q71U36) of alpha Tubulin 1A

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded Breast ca, using alpha Tubulin 1A(107-11148) antibody at 1:250 dilution.

Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
107-11148 diluted at 1:1000

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
alpha Tubulin 1A antibody (107-11148) dilution: 1:500.

alpha Tubulin 1A antibody detects alpha Tubulin 1A protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
alpha Tubulin 1A antibody (107-11148) dilution: 1:500.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using alpha Tubulin 1A(107-11148) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: JurKat
D: Raji
10% SDS PAGE
107-11148 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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