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Anti-alpha Tubulin Antibody
Catalog #: 107-10797
alpha Tubulin antibody, 0.81 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 10376 |
| Protein Name / Synonyms | alpha Tubulin |
| Concentration (lot specific) | 0.81 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | Cytoskeletal Reorganization |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
K-ALPHA-1 antibody, TUBA1B antibody, alpha-tubulin ubiquitous antibody, alpha tubulin antibody, tubulin K-alpha-1 antibody, tubulin alpha-ubiquitous chain antibody, "tubulin, alpha, ubiquitous antibody", tubulin alpha-1B chain antibody, "tubulin, alpha 1b antibody"
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
50 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 118 and 365 (P68363) of alpha Tubulin
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using alpha Tubulin(107-10797) antibody at 1:500 dilution.
Sample (30 ug of whole cell lysate)
A: NIH-3T3
10% SDS PAGE
107-10797 diluted at 1:1000
A: NIH-3T3
10% SDS PAGE
107-10797 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Tubulin alpha-1B(107-10797) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
107-10797 diluted at 1:1000
A: Hela
10% SDS PAGE
107-10797 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.