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Anti-ALYREF Antibody
Catalog #: 144-06010
ALYREF Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q86V81 |
| Gene Id | 10189 |
| Gene Symbols | ALYREF |
| Protein Name / Synonyms | ALYREF, ALY, ALY/REF, BEF, REF, THOC4, THO complex subunit 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
26kDa
Observed MW
30kDa
Immunogen
Recombinant protein of human ALYREF
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using ALYREF antibody (144-06010) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat lung using ALYREF antibody (144-06010) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using ALYREF antibody (144-06010) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat ovary using ALYREF antibody (144-06010) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using ALYREF antibody (144-06010) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using ALYREF antibody (144-06010) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human vermiform appendix using ALYREF antibody (144-06010) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using ALYREF antibody (144-06010) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using ALYREF antibody (144-06010) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using ALYREF antibody (144-06010) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using ALYREF antibody (144-06010) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using ALYREF antibody (144-06010) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using ALYREF antibody (144-06010) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.