Anti-Amphiphysin (N-term) Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	273
Protein Name / Synonyms	Amphiphysin, N-term
Concentration (lot specific)	0.9 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

AMPH1 antibody, AMPH antibody, amphiphysin antibody, amphiphysin I antibody, amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen) antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

76 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 194 of Amphiphysin (Uniprot ID# P49418)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:500.

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:500.

Amphiphysin antibody [N1N2], N-term detects AMPH protein at cytosol on RT2 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded RT2 xenograft.
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:500.

Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis.
A. 50 µg mouse brain lysate/extract
7.5 % SDS-PAGE
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:1000

Amphiphysin antibody [N1N2], N-term detects AMPH protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
5 % SDS-PAGE
Amphiphysin antibody [N1N2], N-term (107-10373) dilution: 1:500

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Amphiphysin(107-10373) antibody at 1:100 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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