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Anti-AMPK alpha 2 Antibody
Catalog #: 107-10385
AMPK alpha 2 antibody, 0.55 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 5563 |
| Protein Name / Synonyms | AMPK alpha 2 |
| Concentration (lot specific) | 0.55 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Immunoprecipitation, Western blot |
| Research Area | AMPK Signaling, Energy Metabolism |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
AMPK antibody, AMPK2 antibody, PRKAA antibody, PRKAA2 antibody, AMPK-alpha-2 chain antibody, 5'-AMP-activated protein kinase, catalytic alpha-2 chain antibody, 5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody, AMPK subunit alpha-2 antibody, protein kinase, AMP-activated, alpha 2 catalytic subunit antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
62 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 29 and 510 of AMPK alpha 2 (Uniprot ID# P54646)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Immunoprecipitation,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000, IP: 1:100-1:500. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using AMPK alpha 2(107-10385) antibody at 1:500 dilution.
AMPK alpha 2 antibody immunoprecipitates AMPK alpha 2 protein in IP experiments. IP Sample: 1000 µg 293T whole cell lysate/extract A. 30 µg 293T whole cell lysate/extract B. Control with 2.5 µg of preimmune rabbit IgG C. Immunoprecipitation of AMPK alpha 2 protein by 2.5 µg of AMPK alpha 2 antibody (107-10385) 10% SDS-PAGE The immunoprecipitated AMPK alpha 2 protein was detected by AMPK alpha 2 antibody (107-10385) diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
AMPK alpha 2 antibody detects PRKAA2 protein by Western blot analysis.
A. 30 µg A431 whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
C. 30 µg A375 whole cell lysate/extract
7.5 % SDS-PAGE
AMPK alpha 2 antibody (107-10385) dilution: 1:1000
A. 30 µg A431 whole cell lysate/extract
B. 30 µg HeLa whole cell lysate/extract
C. 30 µg A375 whole cell lysate/extract
7.5 % SDS-PAGE
AMPK alpha 2 antibody (107-10385) dilution: 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed A431, using AMPK alpha 2(107-10385) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
107-10385 diluted at 1:1000
A:NIH-3T3
7.5% SDS PAGE
107-10385 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.