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Anti-AMPK beta 1 Antibody
Catalog #: 102-26547
Mouse Anti-AMPK beta 1 Antibody, 100 µl
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Mouse |
| Accession Number | Q9Y478 |
| Gene Id | 5564 |
| Gene Symbols | PRKAB1 |
| Protein Name / Synonyms | 1300015D22Rik;5 AMP activated protein kinase subunit beta 1;5''-AMP-activated protein kinase subunit beta-1;AAKB1_HUMAN;AMP-ACTIVATED PROTEIN KINASE, NONCATALYTIC, BETA |
| Clonality | Monoclonal |
| Recommended Applications | Western Blotting, ICC, IP, IHC |
| Research Area | AMPK Signaling, Energy Metabolism |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Other Information
Antigen Type
Recombinant Protein
Images
WB (1:1000)
Western blot detection of AMPK beta 1 in 3T3,Hela,PC-12,COS7 and MDA-MB-468 cell lysates using AMPK beta 1 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.
ICC (1:100)
Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using AMPK beta 1 mouse mAb (dilution 1:100).
IP (1:500)
Immunoprecipitation analysis of Hela cell lysates using AMPK beta 1 mouse mAb.
IHC (1:100)
Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.