Anti-AMPK beta (PRKAB1) (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9Y478
Gene Id	5564
Gene Symbols	PRKAB1
Protein Name / Synonyms	AMPK beta (PRKAB1) (N-term)
Clonality	Polyclonal
Preparation	This AMPK beta (PRKAB1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-34 amino acids from the N-terminal region of human AMPK beta (PRKAB1).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Research Area	AMPK Signaling, Energy Metabolism
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

Specificity

Predicted Reactivity

Rat

Other Names

PRKAB1; AMPK; 5'-AMP-activated protein kinase subunit beta-1

NCBI Accession #

NP_006244.2

Other Accession #

P80386; NP_006244

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

30382

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	The anti-PRKAB1 Pab (Cat# 102-17098) is used in Western blot to detect PRKAB1 in Jurkat cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2).
	Western blot analysis of anti-PRKAB1 Antibody (N-term) (Cat# 102-17098) in mouse heart lysates (35ug/lane). PRKAB1 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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