AMPK gamma 2 antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 51422 |
| Protein Name / Synonyms | AMPK gamma 2 |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Research Area | AMPK Signaling, Energy Metabolism |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
AAKG antibody, AAKG2 antibody, CMH6 antibody, H91620p antibody, WPWS antibody, PRKAG2 antibody, AMPK gamma2 antibody, AMPK subunit gamma-2 antibody, 5'-AMP-activated protein kinase subunit gamma-2 antibody, protein kinase, AMP-activated, gamma 2 non-catalytic subunit antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
63 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 192 and 569 of AMPK gamma 2 (Uniprot ID# Q9UGJ0)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
AMPK gamma 2 antibody detects PRKAG2 protein at cytosol on gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded gastric carcinoma tissue.
AMPK gamma 2 antibody (107-10816) dilution: 1:500.
Sample: Paraffin-embedded gastric carcinoma tissue.
AMPK gamma 2 antibody (107-10816) dilution: 1:500.
Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
107-10816 diluted at 1:1000
A: Mouse brain
10% SDS PAGE
107-10816 diluted at 1:1000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AMPK gamma-2(107-10816) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: Hela S3
10% SDS PAGE
107-10816 diluted at 1:1000
A: Hela S3
10% SDS PAGE
107-10816 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.
