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Anti-AMT (N-term) Antibody
Catalog #: 102-16919
Rabbit Anti-Human AMT (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P48728 |
| Gene Id | 275 |
| Gene Symbols | AMT |
| Protein Name / Synonyms | AMT (N-term) |
| Clonality | Polyclonal |
| Preparation | This AMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-45 amino acids from the N-terminal region of human AMT. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.
Specificity
Predicted Reactivity
Mouse
Other Names
AMT; GCST; Aminomethyltransferase, mitochondrial; Glycine cleavage system T protein
NCBI Accession #
NP_000472.2;NP_001158182.1;NP_001158183.1;NP_001158184.1
Other Accession #
Q8CFA2
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
43946
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of AMT Antibody (N-term) (Cat# 102-16919) in HepG2 cell line lysates (35ug/lane). AMT (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with AMT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | AMT Antibody (N-term) (Cat# 102-16919) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.