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Anti-Amylin Antibody, Biotinylated
Catalog #: RB-08-0028B-50
Rabbit anti-Amylin Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | NP_000406 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from human Amylin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CKCNTATCATQRLANF |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 5-10 µg/ml), Western Blotting (recommended work dilution= 1:1000-2000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Amylin (Islet/insulinoma amyloid polypeptide or IAPP) is a 37-amino acid monomeric polypeptide isolated from pancreatic amyloid. This protein, with a calculated molecular mass of 4 kDa, is a major component of amyloid-rich pancreatic extracts of three type 2 diabetic patients. IAPP has cysteine residues in positions 2 and 7, a feature found in all known calcitonin gene-related peptides. IAPP shows 46% amino acid sequence homology with CGRP II. Amylin is colocalized with somatostatin in endocrine cells of the gastric fundus.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The rabbit anti-human Amylin antibody detects specifically both synthetic peptide and cellular protein from 3T3-L1 cell line. Cross reactivity with mouse and rat are expected from sequence similarity.
References
- Dacquin, R., Davey, R.A., Laplace, C., Levasseur, R., Morris, H.A., Goldring, S.R., Gebre-Medhin, S., Galson, D.L., Zajac, J.D., Karsenty, G. Amylin inhibits bone resorption while the calcitonin receptor controls bone formation in vivo. J. Cell Biol. (2004)
- Janson, J., Soeller, W.C., Roche, P.C., Nelson, R.T., Torchia, A.J., Kreutter, D.K., Butler, P.C. Spontaneous diabetes mellitus in transgenic mice expressing human islet amyloid polypeptide. Proc. Natl. Acad. Sci. U.S.A. (1996)
Expiration:
12 months from the date of shipment when stored properly.