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Anti-Amylin Antibody
Catalog #: RB-08-0007
Rabbit Anti-Human Amylin Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Clonality | Polyclonal |
| Immunogen | Immunogen was peptide near at C-terminus derived from human Amylin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction of immunized serum was purified by Protein G/A affinity chromatography. |
| Immunogen Sequence | CGSPGT(P)PGSRK |
| Recommended Applications | ELISA (recommended work dilution= 1: 5, 000), Western Blotting (recommended work dilution= 1: 1, 000) |
| Reconstitution | Lyophilized rabbit anti-human Amylin antibody is stable at 4°C and below -18°C. Supplied as 200 µg of purified antibody. Reconstitute with sterile water. If 1 ml of water is used, the antibody concentration will be 200 µg/ml.Please avoid freeze-thaw cycles. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Adrenomedullin (AM), alpha- and beta-calcitonin gene-related peptide (CGRP), calcitonin (CT), and amylin are homologous polypeptides with overlapping biological actions such as vasodilatation and inhibition of bone resorption. Amylin (Islet/insulinoma amyloid polypeptide or IAPP) is a 37- amino acid monomeric polypeptide isolated from pancreatic amyloid. This protein, with a calculated molecular mass of 4 kDa, is a major component of amyloid-rich pancreatic extracts of three type 2 diabetic patients. IAPP has cysteine residues in positions 2 and 7, a feature found in all known calcitonin generelated peptides. IAPP shows 46% amino acid sequence homology with CGRP II. Since IAPP has been demonstrated immunochemically in normal beta cells of several mammals, it probably has an important role in respect to pancreatic islet function. The gene which encodes amylin maps to human chromosome 12p12.3-p12.1.
Specificity
Human Amylin polyclonal antibody detects protein derived from human cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
Storage
Lyophilized samples are stable for a year at least from date of receipt when stored at -20° C to -70° C. Upon reconstitution, the antibody can be stored at 2° - 8° C for at least a month without detectable loss of activity. Minimize freezing and thawing.
References
- Dacquin, R., Davey, R.A., Laplace, C., Levasseur, R., Morris, H.A., Goldring, S.R., Gebre-Medhin, S., Galson, D.L., Zajac, J.D., Karsenty, G. Amylin inhibits bone resorption while the calcitonin receptor controls bone formation in vivo. J. Cell Biol. (2004)
- Janson, J., Soeller, W.C., Roche, P.C., Nelson, R.T., Torchia, A.J., Kreutter, D.K., Butler, P.C. Spontaneous diabetes mellitus in transgenic mice expressing human islet amyloid polypeptide. Proc. Natl. Acad. Sci. U.S.A. (1996)
Expiration:
12 months from the date of shipment when stored properly.