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Anti-Androgen Receptor Antibody, Biotinylated
Catalog #: 130-10000B-50
Rabbit anti-Androgen Receptor Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P10275, NP_000035 |
| Gene Id | 367 |
| Gene Symbols | AR DHTR NR3C4 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was full recombinant protein derived from human androgen receptor. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography. |
| Isotype | IgG |
| Recommended Applications | ELISA (recommended work dilution= 1: 40, 000(at least detecting 25 µg/ml)), Western Blotting (recommended work dilution= 1:1, 000-5, 000(at least)), (see image)Immunodetection Analysis: Representative blot from a previous lot. Lane 1.Androgen receptor protein ?g, Lane 2.MCF7 cell lysate , Lane 3.BSA 2?g. The membrane blot was probed with anti-Androgen receptor primary antibody(0.25 µg/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection system. Arrows indicate cellular androgen receptor from human cells (70-80 kDa). |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
The human androgen receptor protein belongs to the class of nuclear receptors called activated class I steroid receptors, which also includes glucocorticoid receptor, progesterone receptor, and mineralocorticoid receptor. These receptors recognize canonical androgen response elements (AREs), which are inverted repeats of 5-prime-TGTTCT-3-prime. The major domains of AR include N- and Cterminal activation domains, which are designated activation function-1 (AF-1) and AF-2, a ligandbinding domain, and a polyglutamine tract. Human androgen receptor contain 920 amino acids and having a molecular mass of 110 kDa.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The rabbit anti-human AR antibody detects specifically target derived from human recombinant protein as well as MCF7 cell lysate at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Related Products
Goat Anti-Androgen Receptor, with HRP-conjugated secondary antibody (cat# ER-04-0011) Recombinant Human Androgen Receptor?Cat# RB-14-0003P
References
- J Trapman J, et al., Cloning, structure and expression of a cDNA encoding the human androgen receptor. Biochem Biophys Res Commun. 1988 May 1;153(1):241-8. PMID: 3377788
Expiration:
12 months from the date of shipment when stored properly.