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Anti-Angiotensin II Antibody
Catalog #: RB-08-0018
Rabbit Anti-Human Angiotensin II Antibody
Product Description
Specifications
| Size | 20 µg, 100 µg, 200 µg, 500 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | CAA77513 |
| Clonality | Polyclonal |
| Immunogen | Immunogen was synthetic peptide derived from Human/Mouse Angiotensin II. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography. |
| Immunogen Sequence | CAAHPFHLGDRVYI |
| Recommended Applications | ELISA (recommended work dilution= 1:10e5), Enzyme Immunoassay(EIA)(recommended work dilution= 2.62 ng/ml) |
| Reconstitution | Supplied as lyophilized purified antibody. The antibody is stable for at least one year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -20°C to -70°C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 4°C for 1 month. If 1 ml of H20 is used, the antibody concentration will be 100 ug/ml. Please avoid multiple freeze-thaw cycles. |
| Research Area | Angiogenesis |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Expiration
12 months from the date of shipment when stored properly.
Description
Angiotensin II (Ang II) is the key peptide hormone in the renin-angiotensin-aldosterone system (RAAS). Its ability to regulate levels of circulating aldosterone relies on actions on adrenal glomerulosa cells. Many of the Ang II effects on glomerulosa cells involve a precisely coordinated regulation of signaling cascades and gene expression. Ang II interacts with its G protein-coupled receptor in zona glomerulosa adrenal cells and triggers a cascade of intracellular signals that regulates steroidogenesis and proliferation. It also promotes vascular smooth muscle cell (VSMC) growth, stimulates Ca(2+)- calmodulin (CaM)-dependent kinase II (CaMKII), and activates cytosolic Ca(2+)-dependent phospholipase A2 (cPLA2), which releases arachidonic acid (AA). ANG II also generates H2O2 and activates Akt, which have been implicated in ANG II actions in VSMC.
Specificity
The rabbit anti-human/mouse Angiotensin II antibody detects specifically target at various concentrations in ELISA and EIA assays. Cross reactivity with rat is expected from sequence similarity
Storage
Store at -20°C. If intended for use within a month, the antibody may be kept at 4°C. Please minimize freezing and thawing cycles.
References
- Schorb, W., et al. 1994. Angiotensin II-induced protein tyrosine phosphorylation in neonatal rat cardiac fibroblasts. J. Biol. Chem. 269: 19626-19632.
- Marrero, M.B., et al. 1995. Direct stimulation of JAK/Stat pathway by the Angiotensin II AT1 receptor. Nature 375: 247-250.
- Yayama, K., et al. 2008. Angiotensin II-induced vasodilation via type 2 receptor: role of Bradykinin and nitric oxide. Int. Immunopharmacol. 8:312-318.