Anti-Angiotensinogen Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	183
Protein Name / Synonyms	Angiotensinogen
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

ANHU antibody, FLJ92595 antibody, FLJ97926 antibody, SERPINA8 antibody, AGT antibody, angiotensin II antibody, angiotensinogen antibody, serpin A8 antibody, angiotensin I antibody, serine (or cysteine) proteinase inhibitor antibody, "alpha-1 antiproteinase, antitrypsin antibody", pre-angiotensinogen antibody, "angiotensinogen (serpin peptidase inhibitor, clade A, member 8) antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

53 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 476 of Angiotensinogen (Uniprot ID# P01019)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HepG2, using Angiotensinogen(107-10407) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma Huh7 xenograft, using AGT(107-10407) antibody at 1:100 dilution.

Sample(30 ug whole cell lysate)
A:Hep G2
10% SDS PAGE
107-10407 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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