Anti-ANKRD45 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	339416
Protein Name / Synonyms	ANKRD45
Concentration (lot specific)	1 mg/ml
Recommended Applications	IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

CT117 antibody, FLJ45235 antibody, MGC161631 antibody, MGC161633 antibody, RP3-436N22.4 antibody, ANKRD45 antibody, ankyrin repeat domain-containing protein 45 antibody, cancer/testis antigen 117 antibody, ankyrin repeat domain 45 antibody

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

32 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 74 and 282 of ankyrin repeat domain 45 (Uniprot ID# Q5TZF3)

Applications

Applications Tested

IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

ANKRD45 antibody detects ANKRD45 protein at cytosol on Normal Liver by immunohistochemical analysis.
Sample: Paraffin-embedded Normal Liver.
ANKRD45 antibody (107-10648) dilution: 1:500.

Sample(30 ug whole cell lysate)
A:Hep G2
12% SDS PAGE
107-10648 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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