Annexin VII antibody, 0.28 mg/ml (100 ul)
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 310 |
| Protein Name / Synonyms | Annexin VII |
| Concentration (lot specific) | 0.28 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
ANX7 antibody, SNX antibody, SYNEXIN antibody, ANXA7 antibody, annexin A7 antibody, annexin-7 antibody, annexin VII antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
53 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 122 and 459 of Annexin VII (Uniprot ID# P20073)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Annexin VII antibody detects ANXA7 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ANXA7 protein stained by Annexin VII antibody (107-10522) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ANXA7 protein stained by Annexin VII antibody (107-10522) diluted at 1:500.
Blue: Hoechst 33343 staining.
Annexin VII antibody detects Annexin VII protein at cytosol on human gastric carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human gastric carcinoma.
Annexin VII antibody (107-10522) dilution: 1:500.
Sample: Paraffin-embedded human gastric carcinoma.
Annexin VII antibody (107-10522) dilution: 1:500.
Annexin VII antibody detects Annexin VII protein by western blot analysis.
A. 30 µg 293T whole cell extract
B. 30 µg A431 whole cell extract
C. 30 µg HeLa whole cell extract
D. 30 µg HepG2 whole cell extract
10 % SDS-PAGE
Annexin VII antibody (107-10522) dilution: 1:1000
A. 30 µg 293T whole cell extract
B. 30 µg A431 whole cell extract
C. 30 µg HeLa whole cell extract
D. 30 µg HepG2 whole cell extract
10 % SDS-PAGE
Annexin VII antibody (107-10522) dilution: 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.