Anti-Anterior Gradient 2 (AGR2) (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O95994
Gene Id	10551
Gene Symbols	AGR2
Protein Name / Synonyms	Anterior Gradient 2 (AGR2) (N-term)
Clonality	Polyclonal
Preparation	This Anterior Gradient 2 (AGR2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 13-42 amino acids from the N-terminal region of human Anterior Gradient 2 (AGR2).
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Anterior gradient 2 (AGR2) is known as a cancer cell marker specifically up-regulated in response to depletion of serum and oxygen. AGR2 has been identified as a tumor marker in primary and secondary cancer lesions, and as a marker for detection of circulating tumor cells (CTCs). Elevated levels of AGR2 are known to increase the metastatic potential of cancer cells, but conditions leading to increased expression of AGR2 are not well understood.

Specificity

Other Names

AGR2; AG2; Anterior gradient protein 2 homolog; HPC8; Secreted cement gland protein XAG-2 homolog

NCBI Accession #

NP_006399.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

19979

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:10-50

	AGR2 Antibody (N-term) (Cat# 102-16719) western blot analysis in HT29,MCF-7,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the AGR2 antibody detected the AGR2 protein (arrow).
	Fluorescent confocal image of A549 cell stained with AGR2 Antibody (N-term)(Cat# 102-16719).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with AGR2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). AGR2 immunoreactivity is localized to Cytoplasm significantly.
	Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with AGR2 antibody (N-term) (Cat# 102-16719), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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