Anti-ANXA7 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P20073
Gene Id	310
Gene Symbols	ANXA7
Protein Name / Synonyms	ANXA7 (Center)
Clonality	Polyclonal
Preparation	This ANXA7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 329-356 amino acids from the Central region of human ANXA7.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. ANXA7 is a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion.

Specificity

Predicted Reactivity

Mouse, Bovine, Monkey

Other Names

ANXA7; ANX7; SNX; Annexin A7; Annexin VII; Annexin-7; Synexin

NCBI Accession #

NP_001147.1;NP_004025.1

Other Accession #

Q07076; Q4R5L5; P20072

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

52739

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	ANXA7 Antibody (Center) (Cat# 102-18009) western blot analysis in Jurkat and SH-SY5Y cell lysates (35ug/lane). This demonstrates that the ANXA7 antibody detected ANXA7 protein (arrow).
	(LEFT)Formalin-fixed and paraffin-embedded human brain tissue reacted with ANXA7 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.(RIGHT)Immunofluorescence analysis of ANXA7 Antibody (Center) with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence. Red counterstaining is PI.
	ANXA7 Antibody (Center) (Cat# 102-18009) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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