Anti-AP1S2 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	8905
Protein Name / Synonyms	AP1S2
Concentration (lot specific)	0.53 mg/ml
Recommended Applications	ICC/IF, Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

MGC:1902 antibody, MRX59 antibody, SIGMA1B antibody, AP1S2 antibody, adapter-related protein complex 1 sigma-1B subunit antibody, sigma 1B subunit of AP-1 clathrin antibody, adaptor protein complex AP-1 sigma-1B subunit antibody, sigma1B-adaptin antibody, clathrin adaptor complex AP1 sigma 1B subunit antibody, golgi adaptor HA1/AP1 adaptin sigma 1B subunit antibody, sigma-adaptin 1B antibody, clathrin assembly protein complex 1 sigma-1B small chain antibody, golgi adaptor HA1/AP1 adaptin sigma-1B subunit antibody, AP-1 complex subunit sigma-2 antibody, "adaptor-related protein complex 1, sigma 2 subunit antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

19 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 157 of AP1S2 (Uniprot ID# P56377)

Applications

Applications Tested

ICC/IF,Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using AP1S2(107-10834) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:2000.

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
107-10834 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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