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Anti-AP2M1 Antibody
Catalog #: 107-10265
AP2M1 antibody, 0.57 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 1173 |
| Protein Name / Synonyms | AP2M1 |
| Concentration (lot specific) | 0.57 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
AP50 antibody, CLAPM1 antibody, mu2 antibody, AP2M1 antibody, "clathrin-associated/assembly/adaptor protein, medium 1 antibody", plasma membrane adaptor AP-2 50kDA protein antibody, AP-2 mu chain antibody, adaptor protein complex AP-2 subunit mu antibody, clathrin coat adaptor protein AP50 antibody, adapter-related protein complex 2 mu subunit antibody, plasma membrane adaptor AP-2 50 kDa protein antibody, clathrin coat-associated protein AP50 antibody, clathrin coat assembly protein AP50 antibody, AP-2 mu 2 chain antibody, AP-2 complex subunit mu antibody, "clathrin adaptor complex AP2, mu subunit antibody", adaptin-mu2 antibody, clathrin assembly protein complex 2 medium chain antibody, HA2 50 kDA subunit antibody, "adaptor-related protein complex 2, mu 1 subunit antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
50 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 150 (Q96CW1) of AP2M1
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded N87 xenograft, using AP2M1(107-10265) antibody at 1:100 dilution.
Sample(30 µg of whole cell lysate)
A:293T
B:HeLa S3
10% SDS PAGE
107-10265 diluted at 1:500
A:293T
B:HeLa S3
10% SDS PAGE
107-10265 diluted at 1:500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.