Apelin (APEL), an endogenous ligand for APJ, is an alternate coreceptor with CD4 for HIV-1 infection. This secreted protein inhibits HIV-1 entry into cells that coexpress APJ and CD4. By proteolytic processing of the peptide precursor, several different active peptides may be produced. Apelin-36, one such inotropic peptide, is being investigated as a potential plasma marker of cardiopulmonary disease. Apelin is highly expressed in brain, mainly in the thalamus, frontal cortex, hypothalamus and midbrain. Apelin is also secreted by the mammary gland into the colostrum and milk. Oral intake of Apelin (in milk and colostrum) may be important in the modulation of the immune responses in neonates and newborns. Apelin has also been found to be a potent stimulator of cardiac contractility and may function in the regulation of the cardiovascular system.

The rabbit anti-human Apelin antibody detects the human recombinant protein and the cellular protein from 3T3-L1 cell lysates at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.

We recommend preparing single-use aliquots of this product. For long term storage, we recommend storing at -20°C after adding an equal volume of 50% glycerol in PBS. When stored in this manner, the antibody should remain active for at least 1 year. Minimize freeze-thaw cycles.