Anti-APEX1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P27695
Gene Id	328
Gene Symbols	APEX1
Protein Name / Synonyms	APEX1, APE, APE1, APEN, APEX, APX, HAP1, REF1, DNA-(apurinic or apyrimidinic site) lyase
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100
IP 1:50 - 1:200

Format

Calculated MW

35kDa

Observed MW

39kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-318 of human APEX1 (NP_542380.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using APEX1 antibody (144-61347).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - [KO Validated] APEX1 Polyclonal Antibody (144-61347)

Western blot analysis of extracts from normal (control) and APEX1 knockout (KO) 293T cells, using APEX1 antibody (144-61347) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Western blot - [KO Validated] APEX1 Polyclonal Antibody (144-61347)

Immunofluorescence analysis of HeLa cells using APEX1 antibody (144-61347). Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (144-61347)

Immunofluorescence analysis of A549 cells using APEX1 antibody (144-61347).
Immunofluorescence - [KO Validated] APEX1 Polyclonal Antibody (144-61347)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug APEX1 antibody (144-61347). Western blot was performed from the immunoprecipitate using APEX1 antibody (144-61347) at a dilition of 1:1000.
Immunoprecipitation - [KO Validated] APEX1 Polyclonal Antibody (144-61347)

Expiration:

12 months from the date of shipment when stored properly.

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