Anti-APG8a/b (MAP1LC3A/B) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9GZQ8
Gene Id	81631
Gene Symbols	MAP1LC3B
Protein Name / Synonyms	APG8a/b(MAP1LC3A/B)
Clonality	Polyclonal
Preparation	This Cleaved-APG8a/b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-125 amino acids from human Cleaved-APG8a/b.
Concentration (lot specific)	0.290 mg/ml
Recommended Applications	IF, Western Blotting, IHC-P
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Specificity

Predicted Reactivity

Bovine

Other Names

MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 light chain 3 beta

NCBI Accession #

NP_073729.1

Other Accession #

O41515; NP_073729.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

14688

Recommended Dilutions

IF: 1:100; WB: 1:1000; IHC: 1:50-100

	Fluorescent image of U251 cells stained with APG8a/b (MAP1LC3A/B) antibody. U251 cells were treated with Chloroquine (50 µM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with 102-10730 APG8a/b (MAP1LC3A/B) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/ml, 5 min). APG8a/b (MAP1LC3A/B) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
	APG8a/b (MAP1LC3A/B) (Cat# 102-10730) western blot analysis in mouse lung tissue lysates (35ug/lane).This demonstrates the MAP1LC3A antibody detected the MAP1LC3A protein (arrow).
	Cleaved-APG8a/b antibody (MAP1LC3A/B) (Cat# 102-10730) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Cleaved-APG8a/b antibody (MAP1LC3A/B) for immunohistochemistry. Clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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