Anti-APG8b (MAP1LC3B) (N-term T29) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9GZQ8
Gene Id	81631
Gene Symbols	MAP1LC3B
Protein Name / Synonyms	APG8b(MAP1LC3B) (N-term T29)
Clonality	Polyclonal
Preparation	This APG8b(MAP1LC3B) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-33 amino acids from the N-terminal region of human APG8b(MAP1LC3B).
Concentration (lot specific)	0.250 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Specificity

Predicted Reactivity

Rat, Bovine

Other Names

MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 light chain 3 beta

NCBI Accession #

NP_073729.1

Other Accession #

A6NCE7; Q62625; Q9CQV6; O41515; NP_073729.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

14688

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	APG8b(MAP1LC3B) Antibody (T29) (Cat# 102-11590) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the APG8b(MAP1LC3B) antibody detected the APG8b(MAP1LC3B) protein (arrow).
	APG8b(MAP1LC3B) Antibody (T29) (Cat# 102-11590) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the APG8b(MAP1LC3B) antibody detected the APG8b(MAP1LC3B) protein (arrow).
	APG8b(MAP1LC3B) Antibody (N-term T29) (Cat# 102-11590)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG8b(MAP1LC3B) Antibody (N-term T29) for immunohistochemistry. Clinical relevance has not been evaluated.
	APG8b(MAP1LC3B) Antibody (N-term T29) (Cat# 102-11590) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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