Anti-APG8b (MAP1LC3B)-T93/Y99 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q9GZQ8
Gene Id	81631
Gene Symbols	MAP1LC3B
Protein Name / Synonyms	APG8b (MAP1LC3B)-T93/Y99 (Center)
Clonality	Polyclonal
Preparation	This APG8b (MAP1LC3B)-T93/Y99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-106 amino acids from the Central region of human APG8b (MAP1LC3B)-T93/Y99.
Concentration (lot specific)	0.330 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Specificity

Other Names

MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 light chain 3 beta

NCBI Accession #

NP_073729.1

Other Accession #

A6NCE7; NP_073729.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

14688

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of APG8b (MAP1LC3B) Antibody (T93/Y99) (Cat# 102-13636) in MCF-7 cell line lysates (35ug/lane). MAP1LC3B (arrow) was detected using the purified Pab.
	APG8b (MAP1LC3B)-T93/Y99 (Cat#AP1802f) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the MAP1LC3B antibody detected MAP1LC3B protein (arrow).
	APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) (Cat# 102-13636) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	APG8b (MAP1LC3B)-T93/Y99 Antibody (Center) (Cat# 102-13636) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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