Synonyms
ARF5 antibody, ADP-ribosylation factor 5 antibody
Specificity
Format
Product Type
Primary antibody
Formulation
1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
21 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 49 and 180 of ARF5
Applications
Applications Tested
ICC/IF,Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed HeLa, using ARF5(107-10484) antibody at 1:500 dilution.

ARF5 antibody detects ARF5 protein at cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ARF5 antibody (107-10484) dilution: 1:500.

Sample(30 µg of whole cell lysate)
A:MOLT4
B:Raji
15% SDS PAGE
107-10484 diluted at 1:1500
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.