Anti-ARFGAP1 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	Q8N6T3
Gene Id	55738
Gene Symbols	ARFGAP1
Protein Name / Synonyms	ARFGAP1 (C-term)
Clonality	Polyclonal
Preparation	This ARFGAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 377-406 amino acids from the C-terminal region of human ARFGAP1.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

ARFGAP1 is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine.

Specificity

Other Names

ARFGAP1; ARF1GAP; ADP-ribosylation factor GTPase-activating protein 1; ADP-ribosylation factor 1 GTPase-activating protein; ARF1-directed GTPase-activating protein

NCBI Accession #

NP_001268411.1;NP_001268412.1;NP_001268413.1;NP_060679.1;NP_783202.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

44668

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100

	Western blot analysis of ARFGAP1 Antibody (C-term) (Cat# 102-15018) against rat recombinant ARFGAP1 (30, 10, and 3 ng/lane, left to right). ARFGAP1(arrow) was detected using the purified Pab. Data courtesy of Dr. Dan Cassel, Department of Biology, Technion, Haifa, Israel.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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