Anti-ARGBP2 (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	O94875
Gene Id	8470
Gene Symbols	SORBS2
Protein Name / Synonyms	ARGBP2 (N-term)
Clonality	Polyclonal
Preparation	This ARGBP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-204 amino acids from the N-terminal region of human ARGBP2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	FC, IHC-P, IF, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Arg and c-Abl represent the mammalian members of the Abelson family of non-receptor protein-tyrosine kinases. They interact with the Arg/Abl binding proteins via the SH3 domains present in the carboxy end of the latter group of proteins. ARGBP2 is the sorbin and SH3 domain containing 2 protein. It has three C-terminal SH3 domains and an N-terminal sorbin homology(SoHo) domain that interacts with lipid raft proteins. The subcellular localization of this protein in epithelial and cardiac muscle cells suggests that it functions as an adapter protein to assemble signaling complexes in stress fibers, and that it is a potential link between Abl family kinases and the actin cytoskeleton.

Specificity

Predicted Reactivity

Mouse, Rat, Pig

Other Names

SORBS2; ARGBP2; KIAA0777; Sorbin and SH3 domain-containing protein 2; Arg/Abl-interacting protein 2; Sorbin

NCBI Accession #

NP_001139142.1;NP_001139143.1;NP_001139144.1;NP_001139145.1;NP_001139146.1;NP_001257700.1;NP_003594.3;NP_066547.1

Other Accession #

O35413; P28220; Q3UTJ2

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

124108

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Fluorescent image of U251 cell stained with ARGBP2 Antibody (N-term)(Cat# 102-17055).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARGBP2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).ARGBP2 immunoreactivity is localized to Nucleus significantly.
	Western blot analysis of ARGBP2 Antibody (N-term) (Cat# 102-17055) in MDA-MB231 cell line lysates (35ug/lane). ARGBP2 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human colon carcinoma reacted with ARGBP2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	Flow cytometric analysis of MDA-231 cells using ARGBP2 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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