Anti-Arginase 1 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Gene Id	383
Protein Name / Synonyms	Arginase 1
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

ARG1 antibody, arginase-1 antibody, type I arginase antibody, liver-type arginase antibody, arginase, liver antibody

Specificity

Species Reactivity

Human, Mouse

Format

Product Type

Primary antibody

Formulation

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

35 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 322 of Arginase I (Uniprot ID# P05089)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:1000-1:50000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Sample (20 ug of whole cell lysate)
A: mouse liver
10% SDS PAGE
107-10825 diluted at 1:50000

Arginase 1 antibody detects ARG1 protein by Western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract
10 % SDS-PAGE
Arginase 1 antibody (107-10825) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using arginase I (107-10825) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using arginase I (107-10825) antibody at 1:500 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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